Journal: Frontiers in Immunology

Article Title: Galectins, Eosinophiles, and Macrophages May Contribute to Schistosoma japonicum Egg-Induced Immunopathology in a Mouse Model

doi: 10.3389/fimmu.2020.00146

Figure Lengend Snippet: Immunohistochemistry for Gal-1 (A) and Gal-3 (B) and mRNA expressions of Gal-1 (C) and Gal-3 (D) in the livers, spleens, and large intestines of S. japonicum- infected mice at 8 weeks p.i. Original magnification 400× (scale bar = 100 μm). There were four mice for immunohistochemistry analysis. For mRNA expression analysis, values are means from triplicate measurements and data are presented as means ± SD; there were eight mice in each group and the data represents from two experiments. * P < 0.05 and ** P < 0.01, S . japonicum -infected mice vs. naive mice.

Article Snippet: After washing with PBS, sections were incubated with rabbit anti-Gal-1 (1:500 dilution; Wuhan Boster Biological Engineering Co., Ltd., Wuhan, China), rabbit anti-Gal-3 polyclonal antibody (IgG1; 1:200 dilution; Bioss, Beijing, China), or rabbit anti-CD68 (1:200 dilution; Wuhan Boster Biological Engineering Co., Ltd.) overnight at 4°C.

Techniques: Immunohistochemistry, Infection, Expressing

Journal: Frontiers in Immunology

Article Title: Galectins, Eosinophiles, and Macrophages May Contribute to Schistosoma japonicum Egg-Induced Immunopathology in a Mouse Model

doi: 10.3389/fimmu.2020.00146

Figure Lengend Snippet: The mRNA expressions of Gal-1, Gal-3, CD86, CD200R, IL-1β, iNOS, Arg1, and Ym1 in peritoneal macrophages of S. japonicum -infected mice at 8 weeks p.i. Values are means from triplicate measurements and data are presented as means ± SD; there were eight mice in each group and the data represents from two experiments. * P < 0.05, ** P < 0.01, and *** P < 0.001, S . japonicum -infected mice vs. naive mice.

Article Snippet: After washing with PBS, sections were incubated with rabbit anti-Gal-1 (1:500 dilution; Wuhan Boster Biological Engineering Co., Ltd., Wuhan, China), rabbit anti-Gal-3 polyclonal antibody (IgG1; 1:200 dilution; Bioss, Beijing, China), or rabbit anti-CD68 (1:200 dilution; Wuhan Boster Biological Engineering Co., Ltd.) overnight at 4°C.

Techniques: Infection

Journal: Frontiers in Immunology

Article Title: Galectins, Eosinophiles, and Macrophages May Contribute to Schistosoma japonicum Egg-Induced Immunopathology in a Mouse Model

doi: 10.3389/fimmu.2020.00146

Figure Lengend Snippet: Correlation analysis between the mRNA expressions of Gal-1/Gal-3 and EPX, Ym1, and CD200R in the livers, large intestines, or peritoneal macrophages of S. japonicum -infected mice at 8 weeks p.i. The r value generates the theoretical line of best fit, and the P value indicates the significance of the correlation. There were eight mice in each group and the data represents from two experiments.

Article Snippet: After washing with PBS, sections were incubated with rabbit anti-Gal-1 (1:500 dilution; Wuhan Boster Biological Engineering Co., Ltd., Wuhan, China), rabbit anti-Gal-3 polyclonal antibody (IgG1; 1:200 dilution; Bioss, Beijing, China), or rabbit anti-CD68 (1:200 dilution; Wuhan Boster Biological Engineering Co., Ltd.) overnight at 4°C.

Techniques: Infection

Journal: Nature Communications

Article Title: Bacterial MgrB peptide activates chemoreceptor Fpr3 in mouse accessory olfactory system and drives avoidance behaviour

doi: 10.1038/s41467-019-12842-x

Figure Lengend Snippet: Generation and validation of ΔFpr3-lacZ mice. a The wild-type Fpr3 allele (top) was mutated by replacement with an expression cassette carrying an FRT site (F1), the lacZ reporter sequence, a loxP site (L1), a neomycin resistance gene ( neo ), second FRT (F2) and loxP sites (L2), and a third loxP site (L3) downstream of the Fpr3 coding exon. Cre -mediated recombination in mice carrying the targeted allele results in deletion of the coding region of Fpr3 and replacement with lacZ . b RT-PCR analyses demonstrate that Fpr3 mRNA of correct size (1023 nt) and sequence is present in the VNO of ΔFpr3-lacZ +/+ and +/− mice but absent in homozygous −/− mice (arrowhead). Fpr3 mRNA is also detected in B6 mice and in mice that are −/− for Gαo or Trpc2. M, DNA size standard in base pairs; H 2 O, template water control. c RNAscope two-colour fluorescence in situ hybridisation for Fpr3 (red) and lacZ (green) in 12-µm coronal sections of VNE. Fpr3 -expressing VSNs are present in the basal VNE layer of B6 and ΔFpr3-lacZ +/− mice but absent in −/− mice. LacZ -labelled VSNs were detected only in +/− and −/− mice. Inset magnifications (bottom) illustrate that VSN somata express either Fpr3 (arrows) or lacZ (asterisks) but not both. DAPI, nuclear counterstain. Scale bars, 100 µm ( c top and d ); 20 µm ( c bottom). d Immunohistochemistry for the β-galactosidase (β-gal) reporter in VSNs of B6, ΔFpr3-lacZ +/− and −/− mice. e Quantification of Fpr3 -positive (red columns) and lacZ -positive (black columns) VSNs (whole VNO, both sites) labelled by RNAscope in the three different genotypes. n ≥ 3 mice per genotype, ANOVA: F (5,19) = 189.1 P < 0.0001; LSD: *** P < 0.001, ns, P = 0.85. Source data are provided as a Source Data file

Article Snippet: Sections were incubated overnight at 4 °C in polyclonal rabbit anti-β-gal primary antibody (5-Prime 3-Prime; Boulder, CO; RRID:AB_2314507) diluted 1:500 in blocking buffer.

Techniques: Biomarker Discovery, Expressing, Sequencing, Reverse Transcription Polymerase Chain Reaction, Control, RNAscope, Fluorescence, In Situ, Hybridization, Immunohistochemistry